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DNA-Extraktions- und -Aufreinigungsset

Lieferant: Q BIOANALYTIC

Beschreibung: Erfolgreiche PCR-Anwendungen hängen von gut gereinigter DNA ab. In der Diagnostik und Analytik von Lebensmitteln und medizinischen Proben haben PCR und Echtzeit-PCR viele Vorteile gegenüber herkömmlichen Methoden. In allen Fällen ist es von Bedeutung, gut gereinigte DNA für nachfolgende PCR-, Echtzeit-PCR-, Hybridisierungs- oder Sequenzierungszwecke zu haben. Gereinigte DNA führt zu einer höheren Sensitivität und auch Spezifität von PCR-basierten Tests in schwierigen Matrizen. Mit QuickBlue Extraktions- und Reinigungskit erhalten Sie immer hohe DNA-Ausbeuten und hochgereinigte DNA. Das Kit verwendet paramagnetische Nanopartikel zur Reinigung. Es kann helfen, DNA aus schwierigen Matrizen wie Nahrung oder sogar Blut im Boden für Anwendungen in forensischen Wissenschaften zu reinigen. DNA in Seewasser kann auch gereinigt werden. Diese Methode vermeidet umfangreiche Zentrifugationsschritte und kann somit in Umgebungen eingesetzt werden, in denen Zentrifugen nicht verwendbar sind. Die Kits enthalten Proteinase K als Reagenz für Zellstörungen, so dass Extraktion und Reinigung mit dem Kit durchgeführt werden können. Die DNA-Ausbeute aus 1 ml Bakterienanreicherung beträgt 10 μg genomische DNA. Das Protokoll kann an automatisierte magnetische Wulstabscheidungsinstrumente angepasst werden.

Dimethylsulfoxid ≥99% Reagenz für die Zellkultur

Lieferant: MP Biomedicals

Beschreibung: Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals.

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

Sicherheitsdatenblätter

β-Nicotinamid-Adenin-Dinucleotid, oxidierte Form (NAD)) ≥98%, weißes Pulver Reagenz für die Zellkultur

Lieferant: MP Biomedicals

Beschreibung: Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.