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Beschreibung: Cas9 mRNA expresses a version of the <em>Streptococcus pyogenes</em> SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest, and the Cas9 protein is employed to perform double-stranded DNA cleavage.

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Beschreibung: T7 RNA Polymerase is a wild-type enzyme to synthesize RNA from DNA templates by <em>in vitro</em> transcription.

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Beschreibung: Ovalbumin (OVA) is a member of the serpin superfamily and the predominant glycoprotein found in egg whites. It is a commonly used antigen for immunisation and biochemical studies and is an established model allergen for airway hyper-responsiveness.

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Beschreibung: <p>mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in <em>Discosoma </em>sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.</p>

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Beschreibung: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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Beschreibung: TriLink® N1-Methyl-2'-O-Methylpseudouridine-5'-Triphosphate

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Beschreibung: NLS-Cre Recombinase mRNA is a capped and polyadenylated messenger RNA encoding Cre recombinase fused to a nuclear localisation sequence (NLS). Cre recombinase is a tyrosine recombinase that catalyses recombination between two loxP sites.

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Beschreibung: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Beschreibung: This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Beschreibung: Biotin-16-Aminoallyl-2'-deoxyuridine can be enzymatically incorporated into DNA via PCR, nick translation, primer extension using Klenow(exo-) DNA polymerase, and terminal deoxynucleotidyl transferase (TdT). Biotinylated cDNA can be prepared with this NTP using Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase.

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Beschreibung: TriLink® Cytidine-5'-O-(1-Thiotriphosphate)

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Beschreibung: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Lieferant: TRILINK BIOTECHNOLOGIES

Beschreibung: This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

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Beschreibung: Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

Lieferant: TRILINK BIOTECHNOLOGIES

Beschreibung: The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, <em>Aequorea victoria</em>. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

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Lieferant: TRILINK BIOTECHNOLOGIES

Beschreibung: 2'-Deoxy-P-nucleoside is a degenerate base which behaves as a universal pyrimidine and therefore base pairs with both adenine and guanine. This feature makes it a powerful tool in mutagenesis studies.

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