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Beschreibung: <p><span style="color:black">This construct expresses a firefly luciferase (FLuc) protein, originally isolated from the firefly <span style="color:black"><em>Photinus pyralis</em></span><span style="color:black">, as self-amplifying RNA (saRNA). FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.</span></span></p>

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Lieferant: TriLink BioTechnologies

Beschreibung: 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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Beschreibung: TriLink® 2'-Deoxyguanosine-5'-O-(1-Thiotriphosphate)

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Beschreibung: TriLink® N1-Propylpseudouridine-5'-Triphosphate

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Beschreibung: TriLink® 2'-Deoxyadenosine-5'-O-(1-Thiotriphosphate)

Lieferant: TriLink BioTechnologies

Beschreibung: 2'-Fluoro NTPs are being utilised in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve <em>in vivo</em> stability.

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Beschreibung: TriLink® 2'-Deoxycytidine-5'-O-(1-Thiotriphosphate)

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Lieferant: TriLink BioTechnologies

Beschreibung: 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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Lieferant: TriLink BioTechnologies

Beschreibung: 5-Propargylamino-2'-deoxyuridine can be enzymatically incorporated into DNA and cDNA to introduce primary amine groups. Primary amines can be labeled with NHS ester fluors and haptens for detection and immobilisation of the resulting DNA strand.

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Beschreibung: TriLink® 7-Deaza-7-Propargylamino-2'-deoxyguanosine-5'-Triphosphate

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Lieferant: TriLink BioTechnologies

Beschreibung: This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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Beschreibung: Cyanine 5-Aminoallyluridine-5'-Triphosphate is a substrate for T7 RNA polymerase, allowing the incorporation of cyanine 5 into RNA and mRNA during <em>in vitro</em> transcription. Cyanine 5 incorporation allows for fluorescent detection of RNA and mRNA independent of protein translation.

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Beschreibung: TriLink® 2'-O-Methyl-N6-Methyladenosine-5'-Triphosphate

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Lieferant: TriLink BioTechnologies

Beschreibung: <p><span style="color:black">This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish <span style="color:black"><em>Aequorea victoria</em></span><span style="color:black">, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.</span></span></p>

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Lieferant: TriLink BioTechnologies

Beschreibung: β-galactosidase (β-gal), a protein product of the bacterial LacZ gene, catalyzes the conversion of β-galactosides into monosaccharides. β-gal is a common marker gene used to assess transfection efficiency.

Lieferant: TriLink BioTechnologies

Beschreibung: TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate

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