Sie suchten nach: Thermo Fisher Scientific

Lieferant: Thermo Fisher Scientific

Beschreibung: Phenol-chloroform, is phenol supplied premixed with chloroform:isoamyl alcohol (25:24:1) and accompanying bottle of buffer to equilibrate at either pH 6,7 or pH 8,0.

Artikel-Nr: (PIER28100)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 28100

Beschreibung: Thermo Scientific Pierce PMPI is a maleimide-and-isocyanate crosslinker for attaching compounds to sulfhydryl groups (cysteines) after conjugating the linker to hydroxyl groups on a molecule by urethane (carbamate) bonds.

UOM: 1 * 50 mg

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Artikel-Nr: (PIER28351)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 28351

Beschreibung: Octylthioglucoside (OTG) is a low-molecular weight non ionic detergent that is used for cell lysis and non denaturing protein solubilisation.

UOM: 1 * 5 g

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Artikel-Nr: (PIER89806)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 89806

Beschreibung: The Protein Stabilising Cocktail is a versatile stabilising solution that increases the shelf-life of purified or partially purified proteins during routine storage.

UOM: 1 * 10 mL

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Lieferant: Thermo Fisher Scientific

Beschreibung: Das Glykoprotein-Isolierungskit ermöglicht die Isolierung von Glykoproteinen aus komplexen Mischungen, einschließlich Serum, Gewebe und kultivierten Zelllysaten. Das Verfahren verwendet Concanavilin A (ConA) oder Weizenkeimagglutinin (WGA) als Ligand, um eine bestimmte Klasse von Glykoproteinen aus komplexen Proteinproben zu binden – solche, die alpha-gestrichelte Mannose- oder terminale Glukosereste (im Fall von ConA) und N-Acetylglucosamin-(GlcNAc)-Gruppen oder Sialinsäure (im Fall von WGA) enthalten. Dieses Kit wurde verwendet, um Glykoproteine ​​aus Serum sowie HeLa- und CHO-Zelllysaten in etwa 50 Minuten zu isolieren.

Artikel-Nr: (PIER44999)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 44999

Beschreibung: Immobilisation Kit for Peptides, SulfoLink™ is a kit containing all the reagents for preparing the peptide sample for efficient immobilisation and columns containing SulfoLink™ resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

UOM: 1 * 1 KIT

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Artikel-Nr: (78840.)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 78840

Beschreibung: This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Pierce™ microcentrifuge tubes are convenient tools for manipulating small volumes of affinity supports for protein purification.

Artikel-Nr: (PIER89801)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 89801

Beschreibung: The Mitochondria Isolation Kit for Tissue enables isolation of intact mitochondria from soft and hard tissue samples in about an hour.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: The Zeba™ spin desalting plates are polypropylene devices containing high-performance Zeba™ desalting resin, offering rapid protein desalting with high recovery of proteins >7000 daltons. Zeba™ desalting devices provide trouble-free desalting and buffer exchange for sample volumes ranging from 2 µl to 4 ml. The 96-well spin plate format enables more high-throughput processing. Zeba™ 96-well spin desalting plates require no resin dispensing or hydration and provide the same high protein recovery as spin columns with at least 95% removal of salts and other small molecules.

Artikel-Nr: (PIER77662)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 77662

Beschreibung: Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.

UOM: 1 * 1 ST

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Lieferant: Thermo Fisher Scientific

Beschreibung: The amine group is the most common target for modifying protein molecules because it is abundant in the majority of proteins either due to the presence of lysine bearing amino side chain functionality or the N-terminal-amine. Amine-reactive biotinylation reagents can be divided into two groups based on water solubility: NHS-esters and sulfo-NHS-esters.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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Artikel-Nr: (PIER80370)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 80370

Beschreibung: (3-Aminopropyl)triethoxysilan

UOM: 1 * 100 g

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Lieferant: Thermo Fisher Scientific

Beschreibung: Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Lieferant: Thermo Fisher Scientific

Beschreibung: NHS-activated agarose is an amine-reactive, beaded-agarose resin for rapid and stable immobilisation of proteins, peptides and other ligands via primary amines.