Sie suchten nach: Merck Millipore (Novagen)

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

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Lieferant: Merck Millipore (Novagen)

Beschreibung: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Artikel-Nr: (71978-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71978-3

Beschreibung: The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots,<i> in situ</i> hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant <i>Thermus thermophilus</i> (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

UOM: 1 * 1 KIT

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Lieferant: Merck Millipore (Novagen)

Beschreibung: Die Perfect DNA™ Leitern enthalten verschiedene DNA-Arten in praktischen, einprägsamen Größen für die Agarose-Gelanalyse. Die Marker weisen einheitliche Bandenintensitäten auf und decken eine große Bandbreite von DNA-Größen ab.

Lieferant: Merck Millipore (Novagen)

Beschreibung: GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die KOD HiFi DNA-Polymerase ist ein einzigartiges Proofreading-Enzym, das aus dem extrem thermophilen <i>Thermococcus kodakaraensis</i> KOD1 isoliert wird (mit höherer Aktivität und Genauigkeit) und somit eine schnellere und genauere PCR-Amplifizierung ermöglicht, als sie mit herkömmlichen Enzymen erreicht werden kann.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Pellet Paint® NF Co-Precipitant ist ein nicht fluoreszierender, mit Farbstoff markierter Träger, der sich für die fluoreszierende Sequenzierung eignet. Er ermöglicht während der Alkoholfällung der Zyklussequenzierung von Reaktionsprodukten die rasche Entfernung von BigDye® Terminatoren. Zyklussequenzierungsreaktionen können mit Zentrifugationszeiten von 10 Minuten schnell gefällt werden. Der gut sichtbare Träger liefert eine einfache Bestätigung, dass die Fällung stattgefunden hat. Die Produkte der Sequenzierungsreaktion werden effizient pelletiert, und die mit Farbstoff markierten Terminatoren verbleiben bei Verwendung der Standard-Fällungsprotokolle von Applied Biosystems während der Alkoholfällung im Überstandmedium. Die Resuspension von pelletierten Sequenzierungsreaktionsprodukten in deionisiertem Formamid kann durch die Überprüfung der Auflösung der Trägerpellets bestätigt werden.

Artikel-Nr: (71840-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71840-3

Beschreibung: The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents. It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

UOM: 1 * 125 µl

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Artikel-Nr: (69831-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 69831-3

Beschreibung: The Mouse Ig-Primer Set is designed for amplification of immunoglobulin (Ig) variable region cDNAs. Mammalian light- and heavy-chain Igs contain conserved regions adjacent to the hypervariable complementarity defining regions (CDRs). Appropriately designed oligonucleotide primer sets enable these regions to be specifically amplified using PCR, after which the products may be sequenced directly or cloned.

UOM: 1 * 1 SET

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Artikel-Nr: (69750-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 69750-3

Beschreibung: The pET-24a-d(+) vectors carry an N-terminal T7-Tag® sequence plus an optional C-terminal His-Tag® sequence. These vectors differ from pET-21a-d(+) only by their selectable marker (kanamycin vs ampicillin resistance).

UOM: 1 * 10 µG

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Artikel-Nr: (69704-4)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 69704-4

Beschreibung: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

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Lieferant: Merck Millipore (Novagen)

Beschreibung: The pSTBlue-1 AccepTor™ vector kits are designed to simplify the PCR cloning process by using non-proofreading thermostable DNA polymerases (eg. KOD XL polymerase and native and recombinant <i>Taq</i> polymerases), which leave single 3'-dA overhangs on the reaction products. The AccepTor™ vectors enable direct ligation by virtue of single 3'-dU overhangs that anneal efficiently with 3'-dA extensions on PCR products. The dU residues are converted to dT residues<i> in vivo</i> following transformation.

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from <i>E. coli</i> without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Artikel-Nr: (70856-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 70856-3

Beschreibung: Convenient solution for selective degradation of RNA.

UOM: 1 * 1 mL

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Lieferant: Merck Millipore (Novagen)

Beschreibung: D-Tube96™ Dialysers allow convenient, high throughput dialysis of 96 samples simultaneously. The device features the advantages of the D-Tube™ Dialyzer Mini in a 96-tube format. D-Tube Dialysers are easy to handle tubes with dual membranes providing a large surface area for fast, efficient dialysis. The membrane is ultra clean, EDTA-treated, regenerated cellulose that is sulfur and heavy metal-free.

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.