Sie suchten nach: Protein-Extraktion

Lieferant: Merck Millipore (Novagen)

Beschreibung: Das PopCulture® Reagenz ist ein auf Reinigungsmittel basiertes Konzentrat zur direkten Beimischung in E. coli-Kulturen, um ohne Zellernte wirksam Proteine extrahieren zu können. Rekombinante Proteine können direkt im Rohextrakt analysiert oder durch Hinzufügen einer Affinitätsmatrix, Auswaschen des gewünschten Proteinkomplexes aus der Matrix (zur Entfernung des verbrauchten Kulturmediums und von Zellverunreinigungen) und Elution des gereinigten Proteins aus der Matrix gereinigt werden. Das Proteinaufreinigungsverfahren mit PopCulture ist ideal zur automatisierten Verarbeitung großer Probenmengen in der Proteomikforschung sowie für jede andere Anwendung, für die eine höhere Geschwindigkeit und praktischere Vorgehensweisen sinnvoll sind.

Lieferant: G-Biosciences

Beschreibung: UPPA PROTEIN-Concentrate™ Kit uses a proprietary reagent UPPA™ (Universal Protein Precipitation Agent) to quantitatively concentrate dilute protein samples as low as 1 ng/ml. Concentration is not affected by the presence of common laboratory agents, including detergents and chaotropes. After precipitation, the sample is washed to remove salts and other interfering agents; complete recovery of sample is produced.

Lieferant: Merck Millipore (Calbiochem‎)

Beschreibung: ProteoExtract® Kits cover the different steps of the proteomics workflow, from protein extraction and abundant protein removal to concentration of protein mixtures and removal of interfering substances, digestion of proteins and selective capturing of phosphopeptides. These kits work in concert with one another to provide protein samples that are directly compatible with downstream analytical applications.

Artikel-Nr: (324708-2)

Lieferant: Merck Millipore (Calbiochem‎)

Hersteller Artikel Nummer : 324708-2

Beschreibung: Native endoproteinase Asp-N from <i>Pseudomonas fragi.</i> Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

UOM: 1 * 2 µG

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Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® Plus Lysonase™ includes BugBuster Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimised, ready-to-use blend of rLysozyme™ and Benzonase® Nuclease in a single kit. This powerful combination of two reagents allows maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. The BugBuster® and rLysozyme™ efficiently lyse the bacterial cells while the Benzonase® degrades nucleic acids to facilitate downstream processing. Requires 5 ml BugBuster and 10 µl Lysonase per 1 g cell paste. The kits provide sufficient reagents for protein extraction from 20 g or 100 g cell paste.

Artikel-Nr: (786-126)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-126

Beschreibung: The Column-PROTEIN-Concentrate™ Kit has been specifically developed for concentration of those proteins that cannot be concentrated either by precipitation or other techniques. The kit is based on a proprietary Protein Binding Resin that binds and immobilises any protein in a low salt buffer between pH 2 to 12. The binding capacity is ~0,5 mg protein/ml Protein Binding Resin.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-168)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-168

Beschreibung: Fraction-FOCUS™ utilises proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins. Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.

UOM: 1 * 10 Tests

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Artikel-Nr: (PIER77662)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : 77662

Beschreibung: Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.

UOM: 1 * 1 ST

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Artikel-Nr: (69022-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 69022-3

Beschreibung: The thrombin cleavage capture kit is designed for cleavage of fusion proteins followed by convenient and quantitative removal of thrombin protease. The method is based on the use of biotinylated thrombin for digestion and its subsequent removal with streptavidin agarose.

UOM: 1 * 1 KIT

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Artikel-Nr: (80-6483-37)

Lieferant: Cytiva

Hersteller Artikel Nummer : 80-6483-37

Beschreibung: Sample grinding kit is ready to use for disrupting small tissue and cell samples for protein extraction.

UOM: 1 * 1 KIT

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Artikel-Nr: (786-680)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : 786-680

Beschreibung: Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

UOM: 1 * 50 mL

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Lieferant: VARIAN

Beschreibung: Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.