Sie suchten nach: Merck+Millipore+(Novagen)

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Lieferant: Merck Millipore (Novagen)

Beschreibung: A common method for transformation of plasmids into E.coli is the use of chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility, and convenience are achieved using Novagen® prepared competent cells. Every Novagen® competent cell strain is verified for phenotype and purity, and is guaranteed for transformation efficiency.

Lieferant: Merck Millipore (Novagen)

Beschreibung: pET-41 series is designed for cloning and high-level expression of peptide sequences fused with the 220 aa GST•Tag™ protein. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand.

Artikel-Nr: (72181-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 72181-3

Beschreibung: 293-Free™ transfection reagent has a unique polycationic liposomal formulation and is designed expressly for transfection of HEK 293 cells grown in suspension culture. It is ideal for mammalian protein production. 293-Free™ transfection reagent is provided as a sterile, ready to use solution. The 1 ml size provides enough reagent to transfect a litre of culture.

UOM: 1 * 1 mL

Sale

Lieferant: Merck Millipore (Novagen)

Beschreibung: Das Proteinextraktionsreagenz BugBuster® ist für den sanften Aufschluss der Zellwand von E. coli  konzipiert, um lösliche Proteine freizusetzen. Es bietet eine einfache, schnelle und kostengünstige Alternative zu mechanischen Methoden wie French Press oder Sonication für die Freisetzung von ausgedrücktem Zielprotein, in Vorbereitung für die Aufreinigung oder sonstige Anwendungen. Die Formulierung verwendet eine Reinigungsmittelmischung, die die Zellwandperforation ohne eine Denaturierung von löslichem Protein ermöglicht.

Artikel-Nr: (71285-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71285-3

Beschreibung: BCA Protein Assay is a simple and reliable protein quantification method. It can be used to determine protein concentration in the range of 20-2000 µg/ml in either a standard assay or microassay configuration.

UOM: 1 * 1 KIT

Sale

Lieferant: Merck Millipore (Novagen)

Beschreibung: Biotinylated Thrombin has covalently attached biotin for easy removal of the enzyme from cleavage reactions using immobilised streptavidin. This preparation is tested for activity using the same assay as for unmodified thrombin, and for >99% binding to streptavidin agarose.

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Artikel-Nr: (71610-3)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71610-3

Beschreibung: D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

UOM: 1 * 1 g

Sale

Lieferant: Merck Millipore (Novagen)

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

Artikel-Nr: (69704-4)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 69704-4

Beschreibung: S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

UOM: 1 * 10 mL

Sale

Lieferant: Merck Millipore (Novagen)

Beschreibung: This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Benzonase® nuclease degrades all forms of DNA and RNA while having no proteolytic activity. Benzonase® is compatible with BugBuster® and PopCulture® protein extraction reagents and therefore can be added to these reagents to eliminate viscosity and remove nucleic acids from <i>E. coli </i>extracts.

Artikel-Nr: (71366-4)

Lieferant: Merck Millipore (Novagen)

Hersteller Artikel Nummer : 71366-4

Beschreibung: The Overnight Express™ Autoinduction Systems enable regulated protein expression in <i>E. coli</i>, without monitoring the culture or adding inducer during cell growth.

UOM: 1 * 1 ST

Sale

Lieferant: Merck Millipore (Novagen)

Beschreibung: BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Lieferant: Merck Millipore (Novagen)

Beschreibung: Die Perfect DNA™ Leitern enthalten verschiedene DNA-Arten in praktischen, einprägsamen Größen für die Agarose-Gelanalyse. Die Marker weisen einheitliche Bandenintensitäten auf und decken eine große Bandbreite von DNA-Größen ab.