This His Tag® monoclonal antibody is a mouse monoclonal antibody (IgG1) directed against the His tag sequence encoded by many of the expression vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context.
- High affinity (Kd = 5 × 10 - 8 to 1 × 10 - 9 M) enables sensitive, specific detection of His tag fusion proteins at antibody concentrations of 0,1 - 0,2 µg/ml. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100 µg package size provides enough purified antibody for 1000 ml of working solution.
Binds to N-terminal, C-terminal and internal His tag sequences
Working dilution: 1:1000 - 1:2000 of antibody working solution
Stability: one year at 2 to 8 °C in lyophilysed form, in solution three months 2 to 8 °C, six months ?20 °C
Type: Primary
Antigen: His tag
Clonality: Monoclonal
Clone:
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Tags
