Neutrophil Elastase Colorimetric Drug Discovery Kit is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays.
Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Bestellinformation: Quantity: 96 assays Contents: Neutrophil elastase (human), (purified) (Prod. No. BML-SE284-9090)(30mU; One U=1μmole/min of MeOSuc-AAPV-pNA at 25 °C, pH 8,0. Purity ≥95% (SDS-Page))Storage: -70°C; avoid freeze/thaw cycles.pNA substrate (MeOSuc-AAPV-pNA) (Prod. No. BML-P213-9090)(60 μl; 20 mM in DMSO)Storage: -70 °CpNA calibration standard (p-nitroaniline) (Prod. No. BML-KI106)(1 ml; 50 μM in assay buffer)Storage: -70 °CElastatinal (inhibitor) (Prod. No. BML-PI103-9090)(10 μl; 20 mM in DMSO)Storage: -70 C Neutrophil elastase assay buffer (Prod. No. BML-KI581)(20 ml; 100 mM HEPES, pH 7,25, 500 mM NaCl, 0,05 % Tween-20; liquid in a screw-cap plastic bottle)Storage: -20 °C or -70 °C 96-well microplate (Prod. No. BML-KI101)Storage: Room temperature
