The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.
- Single proprietary dye provides quantitative measurements of MDR activity in live cells expressed as MDR Activity Factor
- Available in green and gold fluorophores
- Inhibitors included for profiling of specific pumps involved in drug resistance
- Two dye formats allows multiplexing with GFP-expressing cell lines or other CELLESTIAL® dyes
The proprietary AM-ester form of the eFluxx-ID® dye is a hydrophobic non-fluorescent compound that readily penetrates the cell membrane and is subsequently hydrolyzed inside of the cells by intracellular esterases. The resulting probe is a hydrophilic fluorescent dye that is trapped within the cell unless actively pumped out by an ABC transporter. The fluorescence signal of the dye generated within the cells thus depends upon the activity of the ABC transporters. The cells with highly active transporters will demonstrate lower fluorescence because of the active efflux of the probe from the cell. Application of specific inhibitors of the various ABC transporter proteins allows differentiation between the three common types of pumps.
Lieferumfang: Green Multidrug Resistance Assay Kit contains EFLUXX-ID® Green Detection Reagent (1 vial), MDR1 Inhibitor (Verapamil) (300 nmoles), MRP Inhibitor (750 nmoles), BCRP Inhibitor (Novobiocin) (1.5 µmoles), Propidium Iodide (500 µl).
Gold Multidrug Resistance Assay Kit contains EFLUXX-ID® Gold Detection Reagent (2 Vials), MDR1 Inhibitor (Verapamil) (300 nmoles), MRP Inhibitor (750 nmoles), BCRP Inhibitor (Novobiocin) (1.5 µmoles), Propidium Iodide (500 µl).
