HAT activity assay kit

Lieferant: Enzo Life Sciences
ENZOALX850326KI01EA 941 CHF
ENZOALX850326KI01 ALEXALX850326KI01
HAT activity assay kit
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Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.

The kit utilizes active recombinant HAT as a positive control and acetyl-CoA as a cofactor. Acetylation of peptide substrate by active HAT releases the free form of CoA which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous and suitable for kinetic studies. The kit provides a simple, straightforward protocol for a complete assay.

Lieferumfang: Kit includes 2X HAT Assay Buffer, HAT Substrate I, HAT Substrate II, NADH Generating Enzyme, NE (Nuclear Extract 4 mg/ml), HAT Reconstitution Buffer.
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