Sie suchten nach: RT-PCR und cDNA Reagenzien

Lieferant: Quantabio

Beschreibung: qScript™ XLT cDNA SuperMix is a next generation tool for first-strand cDNA synthesis, providing a highly sensitive and easy to use solution for two-step RT-PCR and RT-qPCR. qScript XLT is an engineered M-MLV reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures. Combined with a precise mixture of reaction components, this SuperMix enables superior results over a wide dynamic range of input RNA, with up to 8-fold higher sensitivity than cDNA synthesis kits utilising an RNase H(+) reverse transcriptase (RT).

Sicherheitsdatenblätter

Lieferant: ENZO LIFE SCIENCES

Beschreibung: AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

New Product

Lieferant: ENZO LIFE SCIENCES

Beschreibung: AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

New Product

Lieferant: Quantabio

Beschreibung: Das qScript™ XLT One-Step-RT-PCR-Kit ist ein praktisches und hochempfindliches System für die Verstärkung von RNA-Templates bis zu 2 kb. cDNA-Synthese und PCR-Verstärkung werden im selben Röhrchen ohne Öffnen zwischen den einzelnen Verfahren durchgeführt. Dieses optimierte System bietet maximale RT-PCR-Effizienz, Empfindlichkeit und Spezifität.

Zertifikate

Lieferant: AGILENT

Beschreibung: Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.

Lieferant: Quantabio

Beschreibung: qScript™ cDNA Synthese-Sets enthalten qScript™ Reverse Transkriptase, eine Mischung aus MMLV-Reversen-Transkriptase und einem Ribonuklease-Inhibitorprotein, optimiert für empfindliche und zuverlässige cDNA-Synthese über einen breiten dynamischen Bereich ursprünglicher RNA. cDNA-Synthesereagenzien sind sowohl als SuperMix als auch als Set für spezifische Anwendungen erhältlich.

Sicherheitsdatenblätter Zertifikate

Lieferant: Quantabio

Beschreibung: Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Lieferant: Thermo Fisher Scientific

Beschreibung: The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Sicherheitsdatenblätter

Lieferant: Thermo Fisher Scientific

Beschreibung: The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Zertifikate

Artikel-Nr: (K1612)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : K1612

Beschreibung: The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

UOM: 1 * 1 KIT

Sale

Lieferant: Thermo Fisher Scientific

Beschreibung: Maxima™ Reverse Transcriptase is an advanced enzyme derived by <i>in vitro</i> evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Lieferant: Thermo Fisher Scientific

Beschreibung: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Lieferant: Thermo Fisher Scientific

Beschreibung: RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Zertifikate

Lieferant: Thermo Fisher Scientific

Beschreibung: Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by<i> in vitro</i> evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.