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Beschreibung: The 3.9 antibody reacts with human CD11c, also known as integrin alpha X. This 150 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha X (CD11c) assembles with Integrin beta-2 (CD18) into a receptor known as CR4 which can bind and induce signaling through ICAMs and VCAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

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Beschreibung: The 1A8 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils.

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Beschreibung: The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.

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Beschreibung: The O323 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells.

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Beschreibung: The RPA-2.10 antibody reacts with human CD2, an approximately 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation.

Artikel-Nr: (67-4714-U100)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : 67-4714-U100

Beschreibung: The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies.

UOM: 1 * 100 µG

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Artikel-Nr: (TNB-6600-KIT)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : TNB-6600-KIT

Beschreibung: The fragmentation of genomic DNA by cellular nucleases during the later stages of apoptosis is also one of the most easily measured features of apoptotic cells. Nuclease activity generates DNA fragments ranging from ~300 bp to 50 bp in length, resulting in a typical DNA ‘laddering’ appearance when analyzed by agarose gel electrophoresis. These fragments have exposed 3’-hydroxyl (OH) ends which can be labeled with bromolated deoxyuridine triphosphates (Br-dUTP). An enzyme, terminal deoxynucleotidyl transferase (TdT), is used to catalyze the template-independent addition of Br-dUTP to the 3’-OH ends of double or single stranded DNA. This method is often called TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) or end labeling. Sites where the Br-dUTP is incorporated can then be detected with an antibody specific to BrdU. With the APO-BrdUTM Kit cells are first labeled with Br-dUTP, and then sites of incorporation are detected through staining with a FITC anti-BrdU antibody. Samples can then be analyzed via flow cytometry. Samples that are apoptotic will stain brightly with the anti-BrdU antibody due to the substantial number of exposed 3’-OH sites, while cells that are non-apoptotic will not have incorporated significant amounts of Br-dUTP and will stain dimly. The APO-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

UOM: 1 * 50 Tests

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Artikel-Nr: (TNB-4700-L100)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : TNB-4700-L100

Beschreibung: Lymphocyte Separation Medium (LSM) is a sterile-filtered iso-osmotic solution designed for the in vitro isolation of lymphocytes from diluted whole blood.

UOM: 1 * 100 mL

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Beschreibung: This nucleic acid dye can be used to exclude non viable cells from the analysis of flow cytometry data.

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Artikel-Nr: (TNB-4975-UL100)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : TNB-4975-UL100

Beschreibung: Cell stimulation cocktail is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. It can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.

UOM: 1 * 100 µl

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Lieferant: Tonbo Biosciences

Beschreibung: Ghost Dye Färbelösungen ermöglichen eine klare Unterscheidung von lebenden und toten Zellen für eine präzisere durchflusszytometrische Analyse und sind besonders nützlich für intrazelluläre Färbeprotokolle.

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Lieferant: Tonbo Biosciences

Beschreibung: The UCHT2 antibody is specific for human CD5, a 67 kD transmembrane glycoprotein, that is expressed on most thymocytes, mature T cells, and a subset of B cells. CD5 is involved in modulating antigen receptor signalling in both T and B cells and binds to CD72, expressed on B cells.

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Beschreibung: The 30-H12 antibody reacts with mouse CD90.2 (Thy1.2). CD90.2 is a strain-specific allelic form of the GPI-linked membrane associated protein CD90 and is involved in adhesion and signal transduction. CD90.2 is expressed on thymocytes, mature T cells and neurons in mouse strains that express the CD90.2 allele (BALB/c, CBA, C3H, C57BL/6, SJL and others). 30-H12 does not react with the CD90.1 allele expressed in mouse strains such as PL and AKR.

Artikel-Nr: (70-8067-M002)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : 70-8067-M002

Beschreibung: Goat anti-Rabbit IgG (H&L) - Affinity Pure

UOM: 1 * 2 mg

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Artikel-Nr: (35-8076-M002)

Lieferant: Tonbo Biosciences

Hersteller Artikel Nummer : 35-8076-M002

Beschreibung: Rabbit anti-Mouse IgG (H&L) - Affinity Pure, FITC Conjugate

UOM: 1 * 2 mg

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Lieferant: Tonbo Biosciences

Beschreibung: The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.