Sie suchten nach: Thermo Fisher Scientific

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Lieferant: Thermo Fisher Scientific

Beschreibung: This nuclease-free water is deionized and 0.22 µM membrane-filtered nuclease-free water

Artikel-Nr: (R1181)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : R1181

Beschreibung: Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Artikel-Nr: (SM0271)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : SM0271

Beschreibung: pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

UOM: 1 * 1 KIT

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Artikel-Nr: (FERMR0531)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : R0531

Beschreibung: TurboFect transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

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Lieferant: Thermo Fisher Scientific

Beschreibung: The Maxima™ SYBR® Green/Fluorescein qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. It contains SYBR® Green I dye and is supplemented with fluorescein passive reference dye. Fluorescein is included into the master mix to serve as an internal reference for normalisation
of the SYBR Green I fluorescent signal between different wells to compensate for any instrument or pipetting variation. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG)*. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. The use of Maxima™ SYBR® Green/Fluorescein qPCR Master Mix in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.

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Lieferant: Thermo Fisher Scientific

Beschreibung: Catalyses the transfer of the γ-phosphate from ATP to the 5'-hydroxyl group of single- and double-stranded DNAs and RNAs, oligonucleotides, or nucleoside 3'-monophosphates (forward reaction).

Lieferant: Thermo Fisher Scientific

Beschreibung: GeneJET™ Whole Blood Genomic DNA Purification Mini Kit utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

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Lieferant: Thermo Fisher Scientific

Beschreibung: RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Artikel-Nr: (SM0383)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : SM0383

Beschreibung: MassRuler Low Range DNA Ladder, ready to use, 80 to 1031 bp, is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.

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Artikel-Nr: (ER1751)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : ER1751

Beschreibung: CCNGGA sites and cuts best at 37 °C in Tango buffer.

UOM: 1 * 1 KIT

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Artikel-Nr: (K1441)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : K1441

Beschreibung: 5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

UOM: 1 * 1 KIT

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Artikel-Nr: (SM1821)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : SM1821

Beschreibung: RiboRuler™ high range RNA ladder allows precise sizing of RNA molecules in denaturing agarose gels and Northern blotting.

UOM: 1 * 1 KIT

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