Sie suchten nach: Thermo Fisher Scientific

Artikel-Nr: (EM0821)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : EM0821

Beschreibung: CpG methyltransferase enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context. Thermo Scientific M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37 °C. In addition, the enzyme has been specifically validated for use with genomic DNA – the primary substrate in epigenetic studies.

UOM: 1 * 1 KIT

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Artikel-Nr: (EP0282)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : EP0282

Beschreibung: <i>Taq</i> DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium <i>Thermus aquaticus</i>. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, <i>Taq</i> DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3’-end of PCR products. Native <i>Taq</i> DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in <i>E.coli</i>.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready to use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq™ buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. The green master mix includes density reagent and two tracking dyes for convenient direct loading of PCR products on a gel.

Lieferant: Thermo Fisher Scientific

Beschreibung: ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

Artikel-Nr: (FD0663)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : FD0663

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

Lieferant: Thermo Fisher Scientific

Beschreibung: This nuclease-free water is deionized and 0.22 µM membrane-filtered nuclease-free water

Artikel-Nr: (R1181)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : R1181

Beschreibung: Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Zertifikate

Artikel-Nr: (R0641)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : R0641

Beschreibung: In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA. RNA loading dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis. Formamide also stabilises RNA.

UOM: 1 * 1 KIT

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Artikel-Nr: (FERMR0531)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : R0531

Beschreibung: TurboFect transfection reagent is recommended for efficient transfection of plasmid DNA. Distinct from lipid-based formulas, TurboFect is a sterile solution of a proprietary cationic polymer in water. The polymer forms positively-charged complexes with DNA that are both compact and stable. These complexes protect DNA from degradation and facilitate efficient plasmid delivery into eukaryotic cells.

UOM: 1 * 1 KIT

Sicherheitsdatenblätter Sale

Lieferant: Thermo Fisher Scientific

Beschreibung: T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

Sicherheitsdatenblätter

Artikel-Nr: (SM0271)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : SM0271

Beschreibung: pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.

UOM: 1 * 1 KIT

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Lieferant: Thermo Fisher Scientific

Beschreibung: AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Artikel-Nr: (EN0771)

Lieferant: Thermo Fisher Scientific

Hersteller Artikel Nummer : EN0771

Beschreibung: Thermo Scientific dsDNase is an engineered shrimp DNase designed for rapid and safe removal of contaminating genomic DNA from RNA samples. It is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. Highly specific activity towards double-stranded DNA ensures that RNA and single-stranded DNA, such as cDNA and primers are not cleaved. dsDNase is easily inactivated by moderate heat treatment (55 °C). These features make dsDNase an excellent choice for gDNA elimination prior reverse transcription. It allows for dramatically simplified workflow which combines genomic DNA elimination and cDNA synthesis into one-tube procedure.

UOM: 1 * 1 ST

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