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Artikel-Nr: (BE-414)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-414

Beschreibung: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO243)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 243

Beschreibung: Most molecules have a net charge within a pH range of 2 to 10. When the pH is altered, the net charge on molecules can change drastically. In this experiment, a mixture of 2 chemicals is absorbed onto a solid support ion-exchange column and separated during elution under conditions that influence their net charge.

UOM: 1 * 1 ST

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Artikel-Nr: (EDVO270)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 270

Beschreibung: This experiment introduces students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.

UOM: 1 * 1 ST

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Artikel-Nr: (EDVO212)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 212

Beschreibung: The DNA from bacteriophage Lambda is a well-characterised linear molecule containing six recognition sites for <i>Eco</i> RI (5 distinct sites; 2 are very close in size). In this experiment, Lambda DNA is digested by the <i>Eco</i> RI endonuclease. The digestion products are analysed by agarose gel electrophoresis.

UOM: 1 * 1 KIT

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Artikel-Nr: (BE-308)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-308

Beschreibung: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO957)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 957

Beschreibung: According to the World Health Organisation, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

UOM: 1 * 1 KIT

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Artikel-Nr: (BE-416)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-416

Beschreibung: The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO315)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 315

Beschreibung: Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

UOM: 1 * 1 ST

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Artikel-Nr: (BE-404)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-404

Beschreibung: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO166)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 166

Beschreibung: An infectious outbreak requires prompt and accurate identification of the biological agent. Often, early clinical symptoms are first identified in exposed individuals and then infectious agents are identified by lab tests. In this experiment, students transmit a simulated infectious agent (chemical dye) between classmates which is only visible under long UV light. The pattern of transmission and primary source is documented.

UOM: 1 * 1 ST

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Artikel-Nr: (763-0001)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 330

Beschreibung: In this PCR experiment, students make billions of copies of a small amount of DNA in only 90 minutes. They just need to mix template DNA and primers with PCR beads that contain all of the other components required to carry out a PCR reaction. Students see the increasing amounts of DNA for themselves, taking samples every few cycles and analysing them on a DNA gel.

UOM: 1 * 1 KIT

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Lieferant: EDVOTEK

Beschreibung: Die grundlegenden Konzepte des genetischen Fingerabdrucks werden anhand dieses Ready-to-Load™ Kits erläutert, DNA vom Tatort wird mit der DNA von Verdächtigen verglichen. Fingerabdruckmuster werden mittels Agarose-Gelelektrophorese separiert, und die Studenten erarbeiten, wer das Verbrechen begangen haben könnte.

Artikel-Nr: (BE-408)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-408

Beschreibung: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO253)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 253

Beschreibung: In this experiment, students study the diversity of fish using pre-stained, lyophilised proteins. Total protein from perch, walleye and salmon is extracted and pre-stained using an indicator dye. Each fish protein sample has a characteristic banding pattern when separated by denaturing SDS-polyacrylamide gel electrophoresis, which can be used to identify the specific species.

UOM: 1 * 1 KIT

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Artikel-Nr: (EDVO271)

Lieferant: EDVOTEK

Hersteller Artikel Nummer : 271

Beschreibung: An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient's blood and adding them to a microtitre plate coated with HIV antigen. If HIV antibodies are present, they will bind to the antigens on the plate. In this experiment, students perform an ELISA text by coating microtitre plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

UOM: 1 * 1 ST

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Artikel-Nr: (BE-312)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-312

Beschreibung: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

UOM: 1 * 1 KIT

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