Sie suchten nach: Lehrmittel

Lieferant: G-Biosciences

Beschreibung: Prepacked purification columns for protein purification. Supplied in packs of five.

Artikel-Nr: (BE-416)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-416

Beschreibung: The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-603)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-603

Beschreibung: Dialysis is a routinely used technique in research laboratories to “change” the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as salts. A sample is placed in a bag with a semi-permeable membrane and then placed in the new solution or water. Small molecules, such as salt, can pass through the membrane, moving from an area of high concentration to low concentration. The larger molecules, such as proteins, are retained in the bag. Microdialysis is a modification of dialysis to overcome the problem of dialysing small volumes of precious samples. Microdialysis uses small devices designed for small volumes. This kit allows students to study dialysis using patented microdialysis devices.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-409)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-409

Beschreibung: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-406)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-406

Beschreibung: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-308)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-308

Beschreibung: Following restriction enzyme digestion of DNA molecules, researchers need to rejoin the ends of the DNA to generate recombinant DNA, a process known as ligation. Ligation of DNA is achieved with the bacterial enzyme T4 DNA ligase, which catalyses the formation of phosphodiester bonds. The DNA Ligation kit teaches students about ligation as they ligate several DNA fragments together to make larger pieces of DNA that are easily identified by agarose electrophoresis.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-417)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-417

Beschreibung: The Affinity Chromatography kit teaches the basic principle of affinity chromatography utilising highly specific affinity columns. This lab activity involves preparation of a crude protein extract and running affinity exchange chromatography for isolation of a protein.

UOM: 1 * 1 KIT

Sale

Lieferant: G-Biosciences

Beschreibung: Determination of protein concentration is an essential technique in all aspects of protein studies and proteomics. The Assays for Protein Quantification kit includes three of the most widely used protein assays and allows for a direct comparison of the three assays that teaches students the benefits and limitations of each assay. Each assay is available individually to allow teaching of a specific assay, without the option of comparing and contrasting with other assays. The three assays covered are the Biuret Protein Assay, Lowry Protein Assay and the Coomassie Blue Dye Protein Assay. The Assays for Protein Quantification kit provides all the reagents required to perform both protein assays, including protein standards for accurate quantification, in a single lab activity. An often underestimated factor in quantifying protein is the presence of non-protein interfering agents, such as salts and detergents. This kit teaches students about common laboratory agents that affect the protein assays, the reasoning behind their interferences and how to overcome the interference. Students also learn how to select a protein assay for different applications.

Artikel-Nr: (BE-301)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-301

Beschreibung: Bacterial conjugation is a naturally occurring process that allows the transfer of DNA from one bacterium to another, which allows the transfer of genetic traits, particularly drug resistance. The kit contains two bacteria with different drug resistance genes and all the tools for students to study bacterial conjugation. This kit teaches the difference between bacterial genomic DNA and the transferable plasmid DNA and the mechanisms of bacterial conjugation. Students will also learn important basic microbiological techniques, including bacterial growth in liquid broth and on solid agar plates, antibiotic selection of bacteria and important aseptic techniques.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-405)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-405

Beschreibung: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-403)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-403

Beschreibung: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-414)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-414

Beschreibung: The Size Exclusion Chromatography kit teaches gel filtration or size exclusion chromatography and the use of this method in purification of biological samples. This method is based on separation of protein molecules based on their molecular size. This lab activity involves running size exclusion chromatography for separation of molecules based on their molecular sizes.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (552-0288)

Lieferant: MACHEREY-NAGEL

Hersteller Artikel Nummer : 814300

Beschreibung: Advanced kit for science education.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-312)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-312

Beschreibung: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (BE-501)

Lieferant: G-Biosciences

Hersteller Artikel Nummer : BE-501

Beschreibung: This lab activity is designed to study highly specific lock-key matching properties of antigen-antibody and how this highly specific interaction can be exploited as a tool for research and analysis. This study involves the use of an immunodiffusion technique in which antigen and antibody are allowed to diffuse in a solid agarose medium. When antigen and antibody meet, antigen-antibody complex is formed, which leads to precipitation. Antigen-antibody precipitate is formed in the zone where the concentration of the two matching pairs reaches an optimal known as the zone of equivalence, which results in formation of a visible opaque precipitate region in agarose medium. Those regions of precipitation can be used for determination of concentration or titer of both antigen and antibody. This Antigen-Antibody Interaction kit is a hands-on study of both Ouchterlony Double Diffusion and Radial Immunodiffusion techniques. This kit also provides additional guidance materials for teaching other types of antigen-antibody interactions concepts such as immunoelectrophoresis and immunoprecipitation.

UOM: 1 * 1 KIT

Sale

Artikel-Nr: (552-0287)

Lieferant: MACHEREY-NAGEL

Hersteller Artikel Nummer : 814200

Beschreibung: Advanced kit for science education.

UOM: 1 * 1 ST

Sale