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Product Image-BE-404

Experimentation kits, hydrophobic and hydrophilic proteins

Artikel-Nr: (BE-404)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-404
Beschreibung: This lab activity is designed to demonstrate the different classes of protein molecules and their classification based on solubility. Students learn fractionation of soluble, insoluble membrane proteins, and cytoskeleton proteins from a tissue sample. The insoluble protein fraction is further fractionated into hydrophilic and hydrophobic membrane proteins. Cell membrane structure and the role of hydrophobic membrane proteins are considered. This lab activity also provides an opportunity to understand characteristics of various classes of detergents and the role of detergents in solubilisation of hydrophobic membrane proteins.
UOM: 1 * 1 KIT
Product Image-BE-401

Experimentation kits, physical properties of proteins

Artikel-Nr: (BE-401)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-401
Beschreibung: The Physical Properties of Proteins kit is a lab activity that enables students to investigate the physical properties of several different proteins. Students will learn about protein solubility and how it is affected by various parameters; including temperature, pH, salt and dielectric constant. They will understand about protein precipitation due to pH, high salt and in the presence of organic solvents and about protein denaturation as a result of high temperature. In addition, the kit will demonstrate how non-protein agents, such as detergents drastically alter the physical properties of protein molecules and as a result, understand the importance of detergents in protein solubilisation. This lab activity involves analysis of three different types of pure proteins and then students alter some of those properties with a detergent and re-examine physical properties of those proteins. Students are challenged to consider how physical properties of protein molecules can be exploited for purification and characterisation of proteins and apply their findings on a test sample of complex tissue extract.
UOM: 1 * 1 KIT

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Product Image-BE-409

Experimentation kits, conservation of genetic information

Artikel-Nr: (BE-409)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-409
Beschreibung: In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.
UOM: 1 * 1 KIT
Product Image-BE-312

Experimentation kits, expression of a recombinant protein

Artikel-Nr: (BE-312)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-312
Beschreibung: The final goal in molecular biology is often the expression of a recombinant protein. The transformed plasmids can be used as templates by the bacteria to produce protein. Students learn about essential promoters and other elements necessary for successful protein expression in bacteria, including the differences between inducible and constitutive (unregulated) expression.The Expression of a Recombinant Protein kit allows students to express a protein either constitutively or under the control of an inducible promoter, which is activated with IPTG (isopropyl-beta-D-thiogalactopyranoside).
UOM: 1 * 1 KIT
Product Image-BE-408

Experimentation kits, protein fingerprinting

Artikel-Nr: (BE-408)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-408
Beschreibung: In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of 4 different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.
UOM: 1 * 1 KIT
Product Image-EDVO282

Experimentierkits – Prinzip der Enzymkatalyse

Artikel-Nr: (EDVO282)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : 282
Beschreibung: Anhand dieses einfachen und ungefährlichen Experiments erhalten die Studenten einen Einblick in die Enzymkatalyse, die Merkmale der Enzymaktion sowie die Beziehung zwischen Proteinstruktur und -funktion. Die Studenten führen einen Enzym-Assay durch und bestimmen die Rate der enzymatischen Reaktion.
UOM: 1 * 1 KIT
Product Image-BE-318

Experimentation kits, nucleic acid quantification

Artikel-Nr: (BE-318)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-318
Beschreibung: The kit utilises the principle of diffusion of nucleic acids on a nylon membrane to determine their concentration. No spectrophotometers required.
UOM: 1 * 1 KIT
Product Image-BE-405

Experimentation kits, enzyme analysis

Artikel-Nr: (BE-405)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-405
Beschreibung: This lab activity involves analysis of an enzyme reaction using a specific enzyme substrate and inhibitor. Students study how the rate of enzyme reaction is dependent on substrate concentration and the influence of agents (inhibitors) and conditions that affects the enzyme reaction such as pH and temperature.
UOM: 1 * 1 KIT
Product Image-BE-407

Experimentation kits, protein structure analysis

Artikel-Nr: (BE-407)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-407
Beschreibung: This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.
UOM: 1 * 1 KIT
Product Image-BE-403

Experimentation kits: biodiversity study and biomass analysis

Artikel-Nr: (BE-403)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-403
Beschreibung: Biodiversity lab activity is designed for determination of protein contents and biomass in diverse biological samples to study how biomass is related to biodiversity in nature. Students collect and catalogue plant leaf samples from a diverse group of locally available plants. This lab activity involves determination of natural weight of each plant sample, grinding a predetermined amount of each sample, and the subsequent extraction of proteins from the samples. Students then learn to determine protein contents of each plant sample and attempt to relate the protein content with biomass. Students in this lab activity are challenged to think, analyse, and seek answers as to why protein biomasses vary for a given natural weight for different plants. Finally, they will relate that finding to the biodiversity of nature.
UOM: 1 * 1 KIT
Product Image-EDVOS-47

Linking food science to biotechnology kit

Artikel-Nr: (EDVOS-47)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : S-47
Beschreibung: Investigate how agarose gel electrophoresis unlocks the color code used by food scientists to make colorful candies.
UOM: 1 * 1 KIT
Product Image-EDVO266

What's in my lunch? quantitative milk allergy ELISA kit

Artikel-Nr: (EDVO266)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : 266
Beschreibung: Milk proteins are the most common food allergens in children. Accurate detection and labeling is vital to inform consumers about potentially dangerous foods.
UOM: 1 * 1 KIT
Product Image-EDVO203

Experimentierkits – Isolierung von chromosomaler DNA aus E. coli

Artikel-Nr: (EDVO203)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : 203
Beschreibung: Isolation of high molecular weight chromosomal DNA is the first step in molecular cloning since it is the source of genes in cells. This experiment provides DNA extraction LyphoCells and reagents for isolating chromosomal DNA from <i>E. coli</i>. After spooling from solution, the DNA can be dissolved and analysed by agarose gel electrophoresis as an optional lab extension activity.
UOM: 1 * 1 KIT
Product Image-BE-406

Experimentation kits, protein electrophoresis

Artikel-Nr: (BE-406)
Lieferant: G-Biosciences
Hersteller Artikel Nummer : BE-406
Beschreibung: Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.
UOM: 1 * 1 KIT
Product Image-EDVO302

Experimentierkits, Aufreinigung des Restriktionsenzyms EcoRI

Artikel-Nr: (EDVO302)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : 302
Beschreibung: In this experiment, students purify the restriction enzyme, <i>Eco </i>RI. This procedure utilised an ion exchange chromatograph step for <i>Eco </i>RI purification. Column fractions are assayed for the enzyme using Lambda DNA and digestion products are identified by gel electrophoresis. Fractions that contain <i>Eco </i>RI are identified and pooled. The total and specific activities are calculated. Recommended for advanced courses.
UOM: 1 * 1 KIT
Product Image-EDVO202

Experimentierkits – Isolierung von Plasmid-DNA mit Miniprep

Artikel-Nr: (EDVO202)
Lieferant: EDVOTEK
Hersteller Artikel Nummer : 202
Beschreibung: Die schnelle Isolierung von Plasmid-DNA in kleinem Maßstab ist ein Routineverfahren für das Screening und die Analyse rekombinanter DNA-Moleküle in Klonierungs- und Subklonierungsversuchen. In diesem Experiment isolieren die Studenten Plasmid-DNA ohne Zuhilfenahme von giftigen Chemikalien wie Phenol oder Chloroform.
UOM: 1 * 1 KIT
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