5-Brom-4-chlor-3-indolylphosphat Dinatriumsalz (BCIP-Na) ≥99%, weißgraues Puder
Lieferant: ICN Biomedicals
A chromogenic substrate for alkaline phosphatase.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP disodium salt is soluble in water. It may be used to prepare a stock solution, which in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color.
Disodium salt is soluble in water (50 mg/ml); insoluble in dimethylformamide.
Formel:
C₈H₄BrClNNa₂O₄P MW: 370,43 g/mol Melting Pt: >300 °C (Lit.) Lager Temperatur: Gefrierschrank |
MDL Number:
MFCD00036757 CAS-Nummer: 102185-33-1 |
Spezifikation Testergebnisse
Identity Test | Passes |
Purity | ≥99% |
FTIR | Conforms to Reference |
Loss on Drying | ≤10% |
UV/VIS: I max (water) | 291 ± 3nm |
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