Acridinorange (Zinkchlorid-Doppelsalz) 85% (Farbstoffgehalt), Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
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Achtung
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M-Clarity™ Qualitätsstufe = MQ200
Acridine orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs). Acridine orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine orange dye has been used to analyse mitochondria and lysosomal content by flow cytometry, characterise multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.
Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine orange staining method has been used to deliver more consistent data in the micronucleus test. It is used a diagnostic assay manufacturing, hematology, histology.
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Formel:
C₁₇H₂₀ClN₃·HCl·½ZnCl₂ MW: 369,96 g/mol Lager Temperatur: Room Temperature |
MDL Number:
MFCD00081043 CAS-Nummer: 10127-02-3 EINECS: 233-353-6 |
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Spezifikation Testergebnisse
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Wavelength | 489 - 495 nm |
| Extinction Coefficient | ≥38000 |
| Wavelength | 285 - 291 nm |
| Extinction Coefficient | ≥18000 |
| Wavelength | 265 - 271 nm |
| Extinction Coefficient | ≥38000 |
| Carbon | ≥46.3% |
| Nitrogen | ≥9.5% |
| ICP Major Analysis Zinc Component | Confirmed |
| Solubility (Turbidity) | Clear |
| Solubility (Colour) | Orange to very dark orange and brown to very dark brown and red-orange and red-brown and orange-brown and very dark red-brown |
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