Acridinorange (Chloridsalz), Hyrdrat ≥98% (durch HPLC), Sigma-Aldrich®
Lieferant: SIGMA ALDRICH MICROSCOPY
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Achtung
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Synonyms:
AO, C.I. 46005, 3,6-Bis(dimethylamino)acridine hydrochloride, Basic Orange 14, N,N,N’,N’-Tetramethyl-3,6-acridinediamine hydrochloride, 3,6-Acridinediamine, Acridine orange hydrochloride
M-Clarity™ quality level = MQ200
Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.
- A rigorously purified zinc-free material suitable for use in flow cytometry
- Acridine Orange hydrochloride hydrate dye has been used to stain intracellular acidic vesicles
- to measure lysosomal membrane permeability (LMP) or autophagy detection
- Fluorescent stain for nucleic acids in agarose and polyacrylamide gels
- Has been used to study apoptosis in various cell culture and tissue models
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Formel:
C₁₇H₂₀ClN₃·H₂O MW: 301,81 g/mol (anhy) Melting Pt: 284...287 °C (lit.) Lager Temperatur: Room Temperature |
MDL Number:
MFCD00150070 CAS-Nummer: 65-61-2 EINECS: 200-614-0 |
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Spezifikation Testergebnisse
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Wave Length, c = 0.005g/L; Water | 489 - 495 nm |
| Extinction Coefficient | Typically ≥14,000 |
| Wave Length | 227 - 233 nm |
| Carbon | Typically 55.0% - 67.5% |
| Nitrogen | Typically 11.0% - 14.0% |
| Purity (HPLC) | ≥98.0 % |
| Zinc | Typically <50ppm Zinc |
| Solubility (Turbidity) | Clear |
| Solubility (Color), c = 0.1%, H2O | Orange to Red-Orange |
| Water (by Karl Fischer) | Typically <20% Water |
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