Methylgrün-Zinkchlorid-Doppelsalz ~85%, Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
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Gefahr
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M-Clarity™ Qualitätsstufe = MQ200
Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.
Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridisation as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos. Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.
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Formel:
C₂₇H₃₅BrClN₃·xZnCl₂ MW: 653,24 g/mol Melting Pt: >300 °C (Lit.) Lager Temperatur: Room Temperature |
MDL Number:
MFCD00151094 CAS-Nummer: 7114-03-6 EINECS: 230-415-4 |
Spezifikation Testergebnisse
| Appearance (Colour) | Red to Very Dark Red and Red-Brown and Brown-Red |
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Wave Length, 0,005 G/L H₂O | 626 - 634 nm |
| Extinction Coefficient | ≥60000 |
| Wave Length | 417 - 425 nm |
| Extinction Coefficient | ≥11100 |
| Wave Length | 311 - 319 nm |
| Extinction Coefficient | ≥16000 |
| Solubility (Colour), C = 1 mg/ml, H₂O | Dark Blue to Very Dark Blue and Dark Blue-Green to Very Dark Blue-Green |
| Dye Content | ≥83 % |
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