3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazoliumbromid 98%, Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
|
Achtung
|
M-Clarity™ Qualitätsstufe = MQ200
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
- Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:
- Proliferation assay
- TNF (tumor necrosis factor) bioassay
|
Formel:
C₁₈H₁₆N₅BrS MW: 414,33 g/mol Melting Pt: (Zers., Lit.) Lager Temperatur: Kühlschrank |
MDL Number:
MFCD00011964 CAS-Nummer: 298-93-1 EINECS: 206-069-5 |
Spezifikation Testergebnisse
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Purity (HPLC) | ≥98.0% |
| Solubility (Turbidity), c = 5 mg/ml (0.5%); Water | Clear |
| Solubility (Colour) | Yellow to Very Dark Yellow and Yellow-Green |
| Recommended Retest Period | 5 years |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...