Karbolfuchsin-Lösung (Ziehl-Neelsen), Sigma-Aldrich®
Lieferant: Merck
|
Gefahr
|
Synonyms:
4-[(4-Amino-m-tolyl)(4-iminocyclohexa-2,5-dien-1-yliden)methyl]-o-toluidiniumchlorid
, 4-(Di(4-amino-3-methylphenyl)methylen)-2,5-cyclohexadien-1-yliden)ammoniumchlorid
, 3,3'-Dimethyl-4,4',4''-triaminotrityliumchlorid
, 4-[(4-Amino-m-tolyl)(4-iminocyclohexa-2,5-dien-1-yliden)methyl]-o-toluidin Hydrochlorid
M-Clarity™ Qualitätsstufe = MQ400
Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB).
The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus also that of the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol (Cat. no. 100327). Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. In this instruction for use Löfföer's methylene blue solution(Cat. no. 101287) is used for counterstaining. Those three solutions are each 500 ml and are contained in the AFB-color modified staining kit (Cat. No.100497).
The 500 ml bottle of Ziehl-Neelsen carbolfuchsin solution provides approx. 250 stainings. Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material. Ziehl-Neelsen carbolfuchsin solution - for microscopy is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
|
Formel:
C₂₁H₂₂ClN₃ MW: 351,88 g/mol Dichte: 0,99 g/cm³ (20 °C) Flash-Pt: 47 °C Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD18071399 CAS-Nummer: 4197-24-4 EINECS: 211-189-6 UN: 1992 ADR: 3 (6.1),III |
|
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...