Phenol ≥99,0% für die Biotechnologie pH 4.5
Lieferant: VWR Chemicals
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Gefahr
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Synonyms:
Carbolsäure
, Hydroxybenzol
, Phenylalkohol
- Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
- Liquid phenols offer a safer, easier alternative to crystalline phenol
- Optimised pH for the purification of RNA
- Long term stability > one year
For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.
Formel:
C₆H₅OH MW: 94,11 g/mol Siedepunkt: 182 °C Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD00002143 CAS-Nummer: 108-95-2 EINECS: 203-632-7 UN: 2821 ADR: 6.1,II |
Spezifikation Testergebnisse
O.D.@330nm | ≤ 0,2 |
O.D.@405nm | ≤ 0,1 |
O.D.@510nm | ≤ 0,1 |
pH (Puffer)@25°C | 4,1 - 4,5 |
Reinheit | ≥ 99 % |
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