Phenol ≥99,0% für die Biotechnologie pH 4.5

Lieferant: VWR Chemicals
Gefahr

Synonyms: CarbolsäureHydroxybenzolPhenylalkohol

0981-400MLEA 322 CHF
0981-400ML 0981-100ML
Phenol ≥99,0% für die Biotechnologie pH 4.5
Phenol

  • Manufactured using exceptionally high purity phenol that is double distilled & packaged under nitrogen
  • Liquid phenols offer a safer, easier alternative to crystalline phenol
  • Optimised pH for the purification of RNA
  • Long term stability > one year


For RNA purification, it is best to use a phenol or phenol:chloroform mixture with a pH of 4.5 to 5.5. Although phenol alone will purify RNA in these extractions, messenger RNA can collect with the DNA at the inter-phase in the complete absence of chloroform. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many applications, a 5:1 ratio of phenol:chloroform is a recommended starting point. The phenol used for extraction protocols must be highly purified and essentially free of contamination by phenolic acid, a product of phenol oxidation.

Formel: C₆H₅OH
MW: 94,11 g/mol
Siedepunkt: 182 °C
Lager Temperatur: Raumtemperatur
MDL Number: MFCD00002143
CAS-Nummer: 108-95-2
EINECS: 203-632-7
UN: 2821
ADR: 6.1,II

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Spezifikation Testergebnisse

O.D.@330nm ≤ 0,2
O.D.@405nm ≤ 0,1
O.D.@510nm ≤ 0,1
pH (Puffer)@25°C 4,1 - 4,5
Reinheit ≥ 99 %

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