B cells have been isolated from mononuclear cells through either positive or negative selection using immunomagnetic cell separation techniques.
In positive selection, the targeted isolation of CD19+ B cells was performed, while in negative selection, other cell types were removed to obtain pure CD19+ B cells. These differentiated procedures provide our B-cell products with high specificity.
Derived from mononuclear cells, CD19+ B cells play a central role in the immune response by responding to potential threats through the release of antibodies specific to antigens such as bacteria and viruses. The expression of CD19+ facilitates the identification of these B-cells.
Cells were collected from from a healthy human donors using permission forms and procedures that have been approved by the Institutional Review Board (IRB).
Storage: For prolonged cell preservation or resuscitation, it is recommended to prepare the cells for extended storage in the vapor phase of liquid nitrogen. Storage of cells in the liquid phase of nitrogen is strongly discouraged to prevent potential cross-contamination risks when removing from storage. Long-term storage under −175 °C and for short-term storage, about one or two weeks at −80 °C is permitted, but it is recommended to minimise this duration to ensure optimal stability. Thawed samples should be used immediately and not stored.
