di-Natrium-4-nitrophenylphosphat Hexahydrat (pNPP), Pulver
Lieferant: ICN Biomedicals
Synonyms:
4-Nitrophenyldihydrogenphosphat Dinatriumsalz Hexahydrat
, ortho-Phosphorsäure(4-nitrophenyl)ester Dinatriumsalz Hexahydrat
, ortho-Phosphorsäuremono(4-nitrophenyl)ester Dinatriumsalz Hexahydrat
, p-Nitrophenylphosphat Dinatriumsalz Hexahydrat
, Phosphatasesubstrat
, pNPP
, 4-Nitrophenylphosphat Dinatriumsalz Hexahydrat
p nitrophenol is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
p-nitrophenol is suitable for use as a substrate for alkaline and acid phosphatase.
Typically a pNPP concentration of 1 mg/ml is used
Soluble in water.
Formel:
C₆H₄NNa₂O₆P·6H₂O MW: 371,144 g/mol Melting Pt: >300 °C (Lit.) Lager Temperatur: Gefrierschrank |
MDL Number:
MFCD00066288 CAS-Nummer: 333338-18-4 EINECS: 224-246-5 |
Spezifikation Testergebnisse
Identity Test | Passes |
Water Content | 27 - 31% |
Free p-Nitrophenol | ≤0,005% |
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