Nitroblau-Tetrazoliumchlorid (NBT) für die Molekularbiologie
Lieferant: ICN Biomedicals
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Achtung
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Synonyms:
5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylen)ditetrazoliumdichlorid
, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylen)bis(2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid)
, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylen)di(2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid)
p-Nitroblue Tetrazolium Chloride is an NADPH-diaphorase substrate that competitively inhibits NOS (nitric oxide synthase) (IC50 = 3-4 μM). In combination with 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and an appropriate reaction buffer, Nitroblue tetrazolium forms a substrate for the colorimetric detection of alkaline phosphatase conjugates in a variety of molecular and cell biology techniques.
p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
- Solubility: Slightly (10 mg/mL) soluble in water.
Storage temperature: + 4 °C
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Formel:
C₄₀H₃₀Cl₂N₁₀O₆ MW: 817,65 g/mol Melting Pt: 189…192 °C (Zers., Lit.) Lager Temperatur: Kühlschrank |
MDL Number:
MFCD00012159 CAS-Nummer: 298-83-9 EINECS: 206-067-4 |
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