Nitroblau-Tetrazoliumchlorid (NBT) ≥90% (durch HPLC), Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
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Achtung
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Synonyms:
5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylen)ditetrazoliumdichlorid
, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylen)bis(2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid)
, 3,3'-(3,3'-Dimethoxy-4,4'-biphenylen)di(2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid)
M-Clarity™ Qualitätsstufe = MQ200
Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.
- Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells
- It has been used as a substrate for western blot detection
- It has been used for the staining of histological tissue sections
- Substrate for dehydrogenases and other oxidases
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Formel:
C₄₀H₃₀Cl₂N₁₀O₆ MW: 817,65 g/mol Melting Pt: 200 °C Lager Temperatur: Kühlschrank |
MDL Number:
MFCD00012159 CAS-Nummer: 298-83-9 EINECS: 206-067-4 |
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Spezifikation Testergebnisse
| Appearance (Colour) | Yellow |
| Appearance (Form) | Powder |
| Infrared spectrum | Conforms to Structure |
| Water (by Karl Fischer) | ≤4.5 % |
| Solvent Content (by GC) | ≤10.0 % |
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Nitrogen Corrected for Water and Solvent |
16.4 - 17.9 % |
| Purity (HPLC) | ≥90.0 % |
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Solubility (Colour) c = 10 mg/ml; Water |
Green-Yellow to Dark Yellow |
| Solubility (Turbidity) | Clear |
| Recommended Retest Period | 3 Years |
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