Histopaque®, steril gefiltert, Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
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Gefahr
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Histopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow.
- Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Sterile-filtered and endotoxin-tested
- Stable at 2 to 8 °C for at least 2 years when protected from light
Histopaque-1077 has been used for:
human lymphocyte antigen (HLA) typing
initial isolation step prior to the enumeration of T, B, and 'null' lymphocytes.
preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)[1]
isolation of human lymphocytes[2]
isolation of breast cancer circulating tumor cells (CTCs) from the whole blood[3]
Histopaque-1077 is used in conjunction with Histopaque-1119 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood
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