The blood DNA extraction Kit is designed for the rapid purification of pure genomic DNA from whole blood, serum, plasma, body fluids and dried blood spots.
- No organic extraction or alcohol precipitation
- Complete removal of contaminants and inhibitors for reliable downstream applications
- Typical yield: 4 to 6 µg genomic DNA
- Elution volume: 50 to 200 µl
- High quality DNA obtained that can be directly used in PCR, Southern, any enzymatic reaction, cloning, etc
The procedure involves the processing of either whole blood or DNA isolated from leukocytes, preceded by the lysis of erythrocytes using the RBC lysis buffer provided in the kit. Then, lysis is completed by incubation of whole blood in a solution containing chaotropic ions in presence of proteinase K at 70 °C.
Appropriate conditions for binding DNA to the silica membrane are created by addition of ethanol to the lysate. Contaminants are removed by efficient washing with two different buffers. Pure genomic DNA is finally eluted under low ionic strength in a slightly alkaline elution buffer and it’s ready to use for subsequent reactions.
These kits are used for rapid purification of high-quality, ready-to-use genomic, bacterial, viral DNA isolation from:
• whole blood (human or animal fresh or frozen)
• whole blood treated with citrate, EDTA and heparin
• serum, plasma, buffy coat, platelets, body fluids, dried blood spots
