Safranin O für die Mikroskopie, Sigma-Aldrich®
Lieferant: SIGMA-ALDRICH MICROSCOPY
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Gefahr
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Synonyms:
Basisrot 2
, 3,7-Diamino-2,8-dimethyl-5-phenylphenaziniumchlorid
M-Clarity™ Qualitätsstufe = MQ200
Safranin O, also known as basic red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalised positive charge, an alternation of aromatic and quinonoid structures, and diazotisable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.
- Safranin O is widely employed as a microbiological stain, especially as a counterstain in gram staining to distinguish gram-positive from gram-negative micro-organisms, and for staining bacterial spores
- It is used to demonstrate glycosaminoglycans in human and animal histological specimens and nuclei
- It is a component of Benda's polychrome stain
- It is a component of various polychromes and lignin stains for botanical staining applications
- It has also been applied for the demonstration of mast cells in cytospin preparations and staining surgical frozen sections
- It is a diagnostic assay manufacturing, hematology, histology, microbiology
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Formel:
C₂₀H₁₉ClN₄ MW: 350,85 g/mol Dichte: 1,447 g/cm³ (22 °C) Lager Temperatur: Room Temperature |
MDL Number:
MFCD00011759 CAS-Nummer: 477-73-6 EINECS: 207-518-8 ADR: 1, |
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Spezifikation Testergebnisse
| Appearance (Colour) | Green to very dark green |
| Appearance (Form) | Powder or crystals |
| Thin layer chromatography | Corresponds to standard |
| Solubility (Colour) | Red to very dark red |
| Solubility (turbidity) | Clear to turbid |
| Solubility (method) | 0.01 g in 10 ml methanol |
| pH-transition | 0.3 |
| Colour | Violet |
| pH-transition | 1 |
| Colour | Red to pink |
| Wavelength (1) (UV) | 525.0 - 535.0 nm |
| Extinction (1%/1 cm) (1) (uv) | ≥ 1000 |
| Solvent (UV) | ETOH 50 % |
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