A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva.
- Measure L-lactate in biological samples such as serum, plasma, whole blood, urine, saliva, and cell culture
- Assay 40 samples in duplicate
- Plate-based fluorometric measurement (ex 530 to 540 nm, em 585 to 595 nm)
L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance between NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH). Monitoring lactate levels is, therefore, a good way to evaluate the balance between tissue oxygen demand and utilisation and is useful when studying cellular and animal physiology. Cayman’s L-Lactate Assay provides a fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilised to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, lactate dehydrogenase catalyses the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analysed with an excitation wavelength of 530 to 540 nm and an emission wavelength of 585 to 595 nm.
