A simple and sensitive tool for assaying glycogen from tissue.
- Measure glycogen from tissue samples
- Assay 20 samples in duplicate
- Assay Range: 2,5 to 40 µg/ml
- Plate-based fluorometric measurement (ex 530 to 540 nm, em 585 to 595 nm)
Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for assaying glycogen from tissue. In this assay, glycogen is hydrolyzed by amyloglucosidase to form β-D-glucose, which is then specifically oxidised to D-glucono-δ-lactone by glucose oxidase forming hydrogen peroxide in the process. Hydrogen peroxide, in the presence of horseradish peroxidase, reacts with 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry to generate the highly fluorescent product resorufin which is measure at an excitation wavelength of 530 to 540 nm and an emission wavelength of 585 to 595 nm.
