Our primary human cells offer unmatched consistency and a more biologically relevant tool than other cell types. Our cells are antibody-free and come from a deep inventory to help researchers with reproducibility and improved human insights.
- Highly proliferative and healthy (~85% viability)
- Antibody free and more biologically relevant
- Offered with high cell count (~1 million cells per vial)
- Well-cited in scientific journals
- Ideal for 2D and 3D cultures, microfluidic devices and co-cultures
Primary Human Brain Pericytes (ACBRI498) were isolated by enzymatic dissociation of normal human cerebral cortex tissue. This cell is antibody free. Each vial will initiate a 25 to 75 cm² cell culture. These primary cells are isolated, frozen and qualified using Cell Systems Certified Media and Reagents--which are specified for use in the research environment. Cell Systems primary cells are exclusively from normal, site-specific human tissue specimen, and available in frozen vial or proliferating flask.
These cells were originated using Cell Systems Complete Serum-Free Medium (SF-4Z0-500), and subsequently grown and passaged in Cell Systems Complete Medium (4Z0-500). They are available at Passage 3 (<12 cumulative population doublings) cryopreserved in Cell Systems Cell Freezing Medium™ (4Z0-705). This vial will initiate a Passage 4 cell culture in a 75 cm² flask. Each vial contains more than 1×106 cells.
Each vial of cells is shipped with vials of Bac-Off® (antibiotic) and CultureBoost™ (animal derived growth factors) or CultureBoost-R™ (human recombinant growth factors) at no additional cost.
Standard Tests:
HIV Serologic Test (donor level HIV AB EIA) -Negative
RPR Syphilis Test - Negative
Hepatitis B (HBV) PCR Test (frozen cells) - Negative
Hepatitis C (HCV) PCR Test (frozen cells) - Negative
Mycoplasma spp. (frozen cells) (ATCC method by CLIA Licensed Clinical Lab) - Negative.
Characterisation:
Cytoplasmic alpha-Actinin Filaments >95% positive by immunofluorescence
Cytoplasmic Desmin Intermediate Filament >95% positive by immunofluorescence
Cytoplasmic uptake of Di-L-Ac-LDL <1% positive by immunofluorescence
CD13 pericyte marker >98% positive byence
a-SMA pericyte marker >98% positive by immunofluorescence
CD31 endothelial cell marker <2% positive by immunofluorescence
Uptake of Dil-Ac-LDL <2% positive by immunofluorescence
MAP2 neuronal marker <2% positive by immunofluorescence
Neurofilament neuronal marker <2% positive by immunofluorescence
S100A4 fibroblast marker <2% positive by immunofluorescence
GFAP astrocyte marker <2% positive by immunofluorescence
GS astrocyte marker <2% positive by immunofluorescence
CD11b microglial marker <2% positive by immunofluorescence
Ibal microglial marker <2% positive by immunofluorescence.
Lieferumfang: Shipped on dry ice.
Bitte beachten For research use only.
