PIPES freie Säure, weißes Pulver
Lieferant: ICN Biomedicals
Synonyms:
2,2'-(Piperazin-1,4-diyl)bis(ethansulfonsäure)
, Piperazin-1,4-bis(2-ethansulfonsäure)
, Piperazin-N,N'-bis-2-ethansulfonsäure
PIPES is a zwitterionic N-substituted aminosulfonic acid "Good" buffer. Buffers can be made by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the sesquisodium salt and the disodium salt or dipotassium salt, titrating to the appropriate pH.
Zwitterionic Buffer, Effective buffering range: 6,1 - 7,5 (Merck Index reports 6,0 to 8,5 ), ΔpK/ΔT: 0,0085, pKa: 6,8 at 25 °C ; 6,66 at 37 °C
PIPES is used to buffer glutaraldehyde fixative solutions when fixing plant and/or animal tissue tissue samples. This will help prevent the loss of lipids. It is used to buffer glutaraldehyde and formaldehyde when fixing fungal zoospores for fluorescence microscopy and electron microscopy.
PIPES free acid is not very water soluble (approximately 1 g/l at 100 °C) but will solubilise in 0,5 M NaOH; PIPES salts are water soluble (usually resulting in clear, colourless solutions).
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Formel:
C₈H₁₈N₂O₆S₂ MW: 302,37 g/mol Siedepunkt: 499 °C (1013 hPa) Melting Pt: 300 °C Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD00006159 CAS-Nummer: 5625-37-6 EINECS: 227-057-6 Merck Index: 12,07633 |
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Spezifikation Testergebnisse
| Identity Test | Passes |
| FTIR | Conforms to Standard |
| UV/Vis: OD260 (0,1 M in 0,2 M NaOH) | ≤0,05 |
| UV/Vis: OD280 (0,1 M in 0,2 M NaOH) | ≤0,05 |
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