MOPS-Na, weißes Pulver
Lieferant: ICN Biomedicals
Synonyms:
Natrium-4-morpholin-1-ylpropylsulfonat
, 3-(N-Morpholino)propansulfonsäure Natriumsalz
, MOPS Natriumsalz
MOPS is used as a biological buffer.
Soluble in water (to approximately 33% w/w - clear, colourless solution).
MOPS is a zwitterionic amino acid which acts as one of the "Good" buffers. It is a structural analog to MES; however, MOPS is a more suitable biological buffer than MES because the pH range is closer to pH 7,4. A buffer using MOPS free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa ±1), using about a half-equivalent of NaOH.
A buffer using MOPS sodium salt can be made by mixing equimolar MOPS free acid and MOPS sodium salt solutions to attain a buffer of the desired pH. Titration of a solution of MOPS sodium salt with HCl results in a solution that will contain NaCl, so the ionic strength will be higher than appropriate for some applications. Concentrations higher than 20 mM should not be used with mammalian cell cultures.
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Formel:
C₇H₁₄NNaO₄S MW: 231,25 g/mol Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD00064350 CAS-Nummer: 71119-22-7 EINECS: 428-420-3 |
Spezifikation Testergebnisse
| pH | 9,0 - 10,5 (1% aq solution) |
| pka | pKa −7,2 at 25 °C |
| Water Content | ≤0,5% |
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