Propidiumiodid 50 ug/mL in PBS
Lieferant: Biotium
Synonyms:
3,8-Diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiodid
Propidium Iodide is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.
- Orange/red liquid
- Store at 4°C and protect from light
- λEx/λEm (DNA-bound) = 535/617 nm
- λEx/λEm (no DNA) = 493/636 nm
Propidium iodide (PI) is a cell impermeable nucleic acid intercalating dye. Because PI is excluded from viable cells, it can be used to distinguish necrotic or late apoptotic cells with damaged plasma membranes from viable cells or early apoptotic cells with intact cell membranes. After entering cells and binding to DNA and RNA, the fluorescence of PI is enhanced 20- to 30-fold. PI can be used in flow cytometry and fluorescence microscopy. PI is also utilized as a counterstain in multicolor fluorescent imaging, but nuclear-specific staining requires digestion of cellular RNA.
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Formel:
C₂₇H₃₄I₂N₄ MW: 668,4 g/mol Lager Temperatur: Kühlschrank |
MDL Number:
MFCD00011921 CAS-Nummer: 25535-16-4 |
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