Dithiothreitol (DTT, Cleland's Reagenz) ≥99,4% für die Biotechnologie

Lieferant: VWR Chemicals
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Synonyms: DTT(R*,R*)-1,4-Dimercapto-2,3-butandiol

0281-25GEA 227 CHF
0281-100G 0281-25G 0281-1KG 0281-5G 0281-2500
Dithiothreitol (DTT, Cleland's Reagenz) ≥99,4% für die Biotechnologie
Dithiothreitol (DTT, Cleland's Reagenz)

An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE.


DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel.


It is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven-fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

Formel: C₄H₁₀O₂S₂
MW: 154,25 g/mol
Siedepunkt: < 125 °C (1013 hPa)
Melting Pt: 42…44 °C
Flash-Pt: >110 °C (offener Tiegel)
Lager Temperatur: Kühlschrank
MDL Number: MFCD00004877
CAS-Nummer: 3483-12-3
EINECS: 222-468-7
https://ch.vwr.com/stibo/web/std.lang.all/98/19/2299819.jpg

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Spezifikation Testergebnisse

Abs.@283nm (0.02M, Wasser) ≤ 0,05
DNasen Keine nachgewiesen
Trocknungsverlust < 0,5 %
Schmelzpunkt 39 - 43 °C
Oxidierted DTT < 0,5 %
Proteasen Keine nachgewiesen
Reinheit (-SH Gehalt) > 99,4 %
RNasen Keine nachgewiesen
Löslichkeit (5%, Wasser) Entspricht

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