Iodacetamid, bräunliches Pulver
Lieferant: ICN Biomedicals
|
Gefahr
|
Synonyms:
Iodessigsäureamid
Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.
Iodoacetamide can react with low molecular weight thiol compounds such as mercaptoethanol and glutathione. The reaction with glutathione is utilized in a titration method for the determination of sulfhydryl groups in different compounds. The alkylation reaction forms stable protein derivatives which will remain intact during further study of the protein.
Iodoacetamide is useful in peptide sequencing and as an irreversible enzyme inhibitor. Some enzymes iodoacetamide will inhibit include (but is not limited to): Alcohol dehydrogenase, Alkaline phosphatase (calf intestinal), beta-Amylase, Cathepsin B, Galactose oxidase, Heme oxygenase, L-Lysine decarboxylase. It will irreversibly inhibit the dephosphorylation of both epidermal growth factor receptor and platelet-derived growth factor receptor by alkylation at the active center of one or several protein tyrosine phosphatases in cell lines. Iodoacetamide will also irreversibly inhibit many cysteine proteases. Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.
Soluble in water (recommended stock solution is 10-100 mM). All solutions should be prepared fresh for each use since solutions are very light sensitive.
|
Formel:
ICH₂CONH₂ MW: 184,96 g/mol Siedepunkt: 266 °C (1013 hPa) Melting Pt: 91…94 °C Lager Temperatur: Kühlschrank |
MDL Number:
MFCD00008028 CAS-Nummer: 144-48-9 EINECS: 205-630-1 UN: 2811 ADR: 6.1,III |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...