NEDDylation kit

Lieferant: Enzo Life Sciences
ALEXBMLUW05900001EA 538 CHF
ALEXBMLUW05900001
NEDDylation kit
Proteinkonjugate und Markierungen
This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.

  • This kit provides sufficient material for 20×20µl reactions
  • Generate NEDD8-E2 thioesters for use in the in vitro NEDDylation of target proteins
  • Demonstrate novel proteins are potential targets for NEDDylation
  • Study NEDD8 activation (E1) and conjugation (E2) steps in the NEDDylation cascade
  • Generate substrates for deNEDDylating enzymes
  • Test proteins for NEDD8 E3 ligase activity
  • Avoid freeze/thaw cycles

Note: This Kit does not contain any NEDD8 E3 ligases.

Lieferumfang: NEDD8 Activating Enzyme Solution (E1), UbcH12 Solution (E2), NEDD8 Solution NEDDylation Buffer Mg-ATP Solution, NEDD8 Antibody Solution, Non-reducing Gel Loading Buffer.
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