Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.
- For use with wild type or mutant E2 enzymes in E1 initiated/mediated reactions
- Contains highly sensitive ubiquitin antibody for detection of ubiquitin and ubiquitin conjugates
Uses:
Activation of Ubiquitin for conjugation to wild type or mutant E2 enzymes (user supplied) via thioester bond formation.
Use of Ub-E2 conjugates produced in the subsequent ubiquitin modification of specific target proteins in presence of a dedicated ubiquitin E3 ligase.
Investigation of ubiquitin activation by E1 activating enzyme.
Substitution of the wild type ubiquitin provided with ubiquitin mutants or derivatives (e.g. biotinylated-ubiquitin, Prod. No. BML-UW8705) allowing their activation and subsequent utility in the ubiquitin cascade.
Bestellinformation: Contains 20X Ubiquitin Activating Enzyme Solution, Ubiquitin Solution, Ubiquitin Antibody Solution, Ubiquitin protein conjugates rabbit PAb, 20X Mg-ATP Solution, 2X Non-reducing Gel Loading Buffer, 10X Ubiquitinylation Buffer.
