Most sensitive (0,043 pmol/ml) and complete colorimetric kit for intra- and extracellular cGMP quantification.
For the quantitative determination of cGMP in cell lysates, culture supernatants, plasma, serum, saliva, tissue, and urine from any species.
This is a colorimetric competitive immunoassay kit for the quantitative determination of extracellular cGMP diluted in buffer or intracellular cGMP in cells or tissues lysed in 0.1 M HCl. 0.1M HCl is used to stop endogenous phosphodiesterase activity and stabilize the released cyclic GMP. The 0.1M HCl treated samples are then analyzed directly in a microtiter plate without extraction, drying and reconstitution. Sensitivity is increased by acetylation (reagents included). Absorbance is read at 405 nm.The ready-to-use liquid color-coded reagents provided reduce error.
Bestellinformation: Contains GxR Microtiter plate, Conjugate, Antibody, Assay buffer 2 concentrate, 0.1 M HCl, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Acetic anhydride, Triethylamine. Alternative size available: 89141-242 (1x96 wells).
