Kovacs Indollösung für die Mikrobiologie

Lieferant: PanReac AppliChem
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APPC252908.1608EA 79.9 CHF
APPC252908.1608
Kovacs Indollösung für die Mikrobiologie
Kovac's indole reagent

A reagent for the detection of indole-positive and indole-negative microorganisms. Some microorganisms can cleave tryptophan which is especially abundant in trypticalle digested peptone to give pyruvic acid, ammonia and indole. Indole then reacts with 4-dimethylaminobenzaldehyde to form a dark red dye. As tryptophan also gives a colour reaction with 4-dimethylaminobenzaldehyde, it must be separated from the indole. This is achieved by selectively extracting indole with butanol.


The strain purity of the organism to be tested must first be established; it is then inoculated into an appropriate culture medium and incubated for 18-24 hours at the optimal incubation temperature. The medium is then covered with a layer of KOVÁCS’ indole reagent of about 0,5 cm. If indole is present the reagent layer turns cherry red in colour after a few minutes.


Bestellinformation: Contains 50 g 4-(Dimethylamino) benzaldehyde, 250 ml hydrochloric acid 37 % and 750 ml 1-butanol.

Formel: (CH₃)₂NC₆H₄CHO
MW: 149,19 g/mol
Lager Temperatur: Kühlschrank
MDL Number: MFCD00003381
CAS-Nummer: 100-10-7
UN: 2920
ADR: 8 (3),II

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