Guanidiniumchlorid ≥99%, weißes kristallines Pulver, ultrarein
Lieferant: ICN Biomedicals
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Achtung
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Synonyms:
Guanadine hydrochloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidine HCI, Guanidine chloride, Guanidinium chloride, Guanidine hydrochloride
Guanidine Hydrochloride is a protein denaturant and thus having an important role in molecular weight determinations.
Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Guanidine HCl is used in the isolation of RNA to dissociate the nucleoprotein into its nucleic acid and protein moieties. It is an inhibitor of RNase. Highly concentrated (6 - 8 M) Guanidine HCl solutions are used to denature native globular proteins. It apparently disrupts hydrogen bonds which hold the protein in its unique structure. However, there also is evidence suggesting that guanidine hydrocholoride may disrupt hydrophobic interactions by promoting the solubility of hydrophobic residues in aqueous solutions.
- Ultra Pure Grade
- Suitable for molecular biology applications
- White Crystalline Powder
- pKa: 13.6 (Lit.)
- Store at Room Temperature (15-30 °C). Store Desiccated.
pH: 4 - 6 (6.0 M aq soln) (Lit.)
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Formel:
NH₂C(=NH)NH₂·HCl MW: 95.53 g/mol Melting Pt: 180…190 °C Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD00013026 CAS-Nummer: 50-01-1 Merck Index: 13,04578 |
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Spezifikation Testergebnisse
| Identity Test | Passes |
| Purity | ≥99% |
| FTIR | Conforms to Standard |
| pH (6.0 M aq soln) | 4-6 |
| OD260 (6.0M aq soln) | ≤0.03 |
| Loss on Drying | ≤1.0% |
| Solubility (6.0 M aq soln) | Clear, Colorless Solution |
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