Diethylpyrocarbonat

Lieferant: ICN Biomedicals

Synonyms: DiethyldicarbonatPyrokohlensäurediethylesterDEPC

ICNA0215090280EA 621 CHF
ICNA0215090280 ICNA0215090225 ICNA0215090205 ICNA0215090250
Diethylpyrocarbonat
Diethylpyrocarbonat

Diethyl Pyrocarbonate (DEPC) is a chemical use to inactivate RNase enzymes and is sensitive to moisture and pH. It is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen.


It is effective as a nuclease inhibitor, modification reagent for His and Tyr residues in proteins. It is used as a robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases. It is used to modify proteins and nucleic acids. It is also used for Dot blot hybridisation, to dilute total RNA isolated from different micro-organisms.


Soluble in alcohols, esters, ketones, hydrocarbons. Slowly soluble in water with hydrolytic decomposition yielding ethanol and CO2.

Formel: O(COOC₂H₅)₂
MW: 162,14 g/mol
Lager Temperatur: Kühlschrank
MDL Number: MFCD00009106
CAS-Nummer: 1609-47-8

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