Clostridium histolyticum Collagenase, Type I, MP Biomedicals
Lieferant: ICN Biomedicals
Collagenase is a protease which cleaves the triple-helical protein called collagen. It has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
- Enzyme Commission Number: E.C.-3.4.24.3
It dissolve at a concentration of 1 mg/ml in 0,05 M TES with 0,36 mM calcium chloride, pH 7,5.
Unit definition: one unit will liberate peptides from collagen equivalent in ninhydrin color to 1,0 μmole of L-leucine in 5 hr at pH 7,4 and 37 °C in presence of calcium ions. Contains clostripain, neutral protease and trypsin activities.
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