Coomassie Brillantblau G 250, dunkles rötlich-violettes Pulver für die Elektrophorese C.I. 42655
Lieferant: ICN Biomedicals
Synonyms:
C.I. 42655
, Coomassie Blau G 250
, Brillantblau G
, Hydrogen[4-[4-(p-ethoxyanilino)-4'-[ethyl(m-sulfonatobenzyl)amino]-2'-methylbenzhydrylen]-3-methylcyclohexa-2,5-dien-1-yliden](ethyl)(m-sulfonatobenzyl)ammonium Mononatriumsalz
, Brillantindocyanin G
, Acid Blue 90
Brilliant blue G is a useful dye for SDS gels as it readily stains proteins with minimal background color. Protein bands can be visualised during staining. It has been used in the Bradford dye-binding protein assay.
A mechanism for dye binding to protein has been proposed, based onmeasuring coomassie brilliant blue G-250 (CBBG) absorbance spectra during titration of the dye reagent in the absence of protein and its response to different polyamino acids. Brilliant blue G has been used for determining critical micelle concentration of detergents. Recent research reveals CBBG as a P2X7 (P2X purinoceptor 7: P2RX7) antagonist and suggests that it exhibits neuroprotective properties in rat spinal cord cells.
Dark blue solution. Soluble in hot water (50 mg/ml), ethanol (40 mg/ml), methanol and 10% trichloroacetic acid.
Formel:
C₄₇H₄₈N₃NaO₇S₂ MW: 854,04 g/mol Lager Temperatur: Raumtemperatur |
MDL Number:
MFCD00078482 CAS-Nummer: 6104-58-1 |
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