Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound

Lieferant: Thermo Fisher Scientific
PIER26133EA 722 CHF
PIER26133
Pegylation reagent, CT(PEG)₁₂ carboxy-PEG-thiol compound
Protein-Aufbereitungs Proteinreinigungs-Reagenzien
Carboxy-PEG12-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

  • Provides carboxylate "handles" for protein immobilisation via EDC crosslinking
  • PEG groups are flexible, non-immunogenic and hydrophilic
  • Terminal monodentate thiol reacts with gold surfaces through dative bonding
  • Terminal carboxylic acid group allows conjugation to amine-containing affinity ligands

The use of MT(PEG)4 with CT(PEG)12 in surface modification can form a hydrophilic "lawn" of methyl ether-terminated PEGs with periodic exposed carboxylic acid-containing PEGs. The exposed carboxylic acid groups can be coupled to affinity ligands using the carbodiimide coupling reaction with EDC and sulpho-NHS.
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