Pierce strong cation exchange and strong anion exchange spin columns use membrane-adsorption as the chromatographic method of choice to fractionate proteins based on their charge differences. The column matrix has a highly porous structure, with pores larger than 3000 nm, giving proteins ready accessibility to the membrane's charged ligands. These adsorptive membranes maintain high efficiencies at high flow rates and during fractionation of large biomolecules with small diffusivities.
- Fast and simple - membrane-based spin format eliminates column packing
- Convenient and expandable - centrifugal format enables convenient processing of multiple samples in parallel
- Robust - membrane adsorber spin columns do not crack or run dry
- Low bed volume - small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions
Realise shorter diffusion times, higher protein recovery, and intact biological activity of eluted samples with Pierce strong cation exchange and strong anion exchange spin columns. Unlike resin-based column chromatography, these columns utilize ion exchange chromatography to separate molecules based on differences in their accessible surface charges.
Realise shorter diffusion times, higher protein recovery, and intact biological activity of eluted samples with Pierce strong cation exchange and strong anion exchange spin columns. Unlike resin-based column chromatography, these columns utilize ion exchange chromatography to separate molecules based on differences in their accessible surface charges.
Strong ion exchange spin column applications
• Pre-fractionation of proteins in lysate
• Scouting purification conditions for new protein preparation protocols
• Removal of endotoxins from monoclonal antibodies
• Polishing His-tagged proteins after metal chelate chromatography
• Purification and concentration of proteins
• Purification of antibodies from serum, ascites, or tissue culture supernatant
• Removal of detergent from protein solutions
• Sample preparation before 1D or 2D PAGE
• Purification of phosphopeptides before MS analysis
Mini format: for binding up to 4 mg (500 µl capacity)
Maxi format: for binding up to 80 mg (20 ml capacity)
Bitte beachten For research use only. Not for use in diagnostic procedures.
