An optimized buffer for aldehyde-hydrazide and aldehyde-alkoxyamine coupling reactions.
- Efficient - maximises coupling efficiency and decreases required coupling reaction incubation time
- Gentle - does not interfere with protein function and is easily removed by desalting or dialysis
- Two-component kit with catalyst and equilibration buffer
The Thermo Scientific GlycoLink Coupling Catalyst decreases reaction times and increases aldehyde-hydrazide coupling efficiency, resulting in greater than 90% coupling of glycoproteins in 4 hours.
The GlycoLink Coupling Catalyst is a two component kit composed of aniline and sodium acetate buffer. The catalyst is also useful for increasing the binding efficiency of oxidized glycoproteins or other carbohydrate-containing molecules to hydrazide resins.
Producing reactive aldehydes via sodium meta-periodate oxidation of cis-diols in glycoproteins is a useful technique for variety of protein labeling and detection methods. The GlycoLink Coupling Catalyst (aniline) increases the efficiency of these reactions by acting as a nucleophilic catalyst that accelerates bond formation through a Schiff base intermediate. The catalyst also reduces the amount (molar excess) of hydrazide or alkoxyamine required in labeling reactions, thereby eliminating the need for DMSO to solubilise high concentrations of labeling reagent.
Lieferumfang: Kit is supplied with GlycoLink coupling buffer, 100 ml and Aniline, 0,2 ml.
